John J Alexander, PhD

Assistant Professor


Molecular Diagnostics

Office: 220

Phone: 404-778-8499


Areas of Specialization

  • General Molecular Diagnostics
  • Molecular Diagnostics for Eye Disorders
  • Development and Evaluation of rAAV Gene Therapy Vectors for Retinal Disorders


  • ABMG Clinical Molecular Genetics Fellowship, Department of Genetics, University of Alabama at Birmingham, 2011-2013
  • Postdoctoral Fellowship, Vision Sciences, University of Alabama at Birmingham, 2009-2011
  • Postdoctoral Fellowship, Pathology, Immunology and Laboratory Medicine, University of Florida College of Medicine, 2007-2009
  • PhD, Medical Science - Genetics, University of Florida College of Medicine, 2002-2007
  • BS, Microbiology and Cell Science, University of Florida, 1993-1999

Board Certifications

American Board of Medical Genetics:  Clinical Molecular Genetics

Professional Memberships

Fellow American College of Medical Genetics (FACMG)


Hsiao MC, Piotrowski A, AlexanderJ, Callens T, Fu C, Mikhail FM, Claes KB, Messiaen L: "Palindrome-Mediated and Replication-Dependent Pathogenic Structural Rearrangements within the NF1 Gene," Hum Mutat. 2014 Apr 23.

Erin Garrigan, Nicole S. Belkin, John J. Alexander, Zhao Han, Fedrica Seydel, Jamal Carter, Mark Atkinson, Clive Wasserfall, Mike J. Clare-Salzler, Matthew A. Amick, and Sally A. Litherland: “Persistent STAT5 Phosphorylation and Epigenetic Dysregulation of GM-CSF and PGS2/COX2 Expression in Type 1 Diabetic Human Monocytes,” 18 Oct 2013 PLOS ONE 10.1371/journal.pone.0076919

Boye SE*, Alexander JJ*, Boye SL, Witherspoon CD, Sandefer KJ, Conlon TJ, Erger K, Sun J, Ryals R, Chiodo VA, Clark ME, Girkin CA, Hauswirth WW, Gamlin PD: “Evaluating the human GRK1 promoter in conjunction with AAV5 as a means of photoreceptor specific expression in primate retina,” Hum Gene Ther. 2012 Oct;23(10):1101-15 *These authors contributed equally to this work

András M. Komáromy, John J. Alexander, Jessica S. Rowlan, Monique M. Garcia, Vince A. Chiodo, Asli Kaya, Jacqueline C. Tanaka, Gregory M. Acland, William W. Hauswirth, Gustavo D. Aguirre: “Gene therapy rescues cone function in congenital achromatopsia,” Human Molecular Genetics, 2010 Jul 1;19(13):2581-93.

Pang J, Boye SE, Lei B, Boye SL, Everhart D, Ryals R, Umino Y, Rohrer B, Alexander J, Li J, Dai X, Li Q, Chang B, Barlow R, Hauswirth WW: “Self-complementary AAV-mediated gene therapy restores cone function and prevents cone degeneration in two models of Rpe65 deficiency," Gene Ther. 2010 Jul;17(7):815-26
Bierschenk L, Alexander J, Wasserfall C, Haller M, Schatz D, Atkinson M: “Vitamin D Levels in Subjects With and Without Type 1 Diabetes Residing in a Solar Rich Environment,” Diabetes Care, November 2009 vol. 32 no. 11 1977-1979.

Parker MJ, Xue S, Alexander JJ, Wasserfall CH, Campbell-Thompson ML, Battaglia M, Gregori S, Mathews CE, Song S, Troutt M, Eisenbeis S, Williams J, Schatz DA, Haller MJ, Atkinson MA: “Immune Depletion with Cellular Mobilization Imparts Immunoregulation and Reverses Autoimmune Diabetes in NOD Mice,” Diabetes, 2009 Oct;58(10):2277-84

Alexander JJ, Hauswirth WW: “Adeno-associated viral vectors and the retina,” Adv Exp Med Biol. 2008;613:121-8

Devid Damiani, John J Alexander, Jason R O'Rourke, Mike McManus, Ashu Jadhav, Constance L Cepko, William W Hauswirth, Brian D Harfe, Enrica Strettoi: “Dicer inactivation leads to progressive functional and structural degeneration of the mouse retina,” Journal of Neuroscience, May 7, 2008, 28(19):4878-4887

AM Komaromy, JJ Alexander, AE Cooper, VA Chiodo, GM Acland, WW Hauswirth and GD Aguirre: “Targeting gene expression to cones with human cone opsin promoters in recombinant AAV,” Gene Therapy, March 13, 2008

J.J. Alexander, Y. Umino, D. Everhart, B. Chang, S.H. Min, Q. Li, A.M. Timmers, N.L. Hawes, J.J. Pang, R.B. Barlow, and W.W. Hauswirth: “Restoration of Cone Vision in a Mouse Model of Achromatopsia,” Nature Medicine 13, 685 - 687 (2007)